epitope tag reporter protein antibodies

Tags and Cell Markers

Tags and cell markers Product epitope tag and fusion protein antibodies in one central location Organelle and cell type markers help visualize structures and cells in your IHC and IF experiments Epitope tag and fusion proteins antibodies detect recombinantly-expressed proteins that have been tagged with a reporter protein Browse all related products Features Organelle markers and dyes

Epitope Tags Reporters

Epitope tags are peptide or protein sequences covalently conjugated usually to the C- or N-terminus of a recombinant protein The addition of epitope tags allows researchers to better identify and purify a target protein within a cell lysate or localize it and assess its expression in intact cells when studying transfectants expressing the fusion protein

Tags and Tag Antibodies

However specific antibodies to a target protein are not always available In that case the target protein can be added a marker (tag) by genetic engineering techniques and expressed in the living organism A specific antibody to the tag can distinguish the target protein from other similar proteins

Identification of Human Single

Wu et al describe the development of a versatile platform for rapid isolation of fully human single-domain antibodies and apply this methodology to identify SARS-CoV-2-specific antibodies These human single-domain antibodies target diverse epitopes within the SARS-CoV-2 spike protein receptor binding domain (RBD) and may yield potential therapeutic candidates for COVID-19

Protein Tagging Technologies: New Ways to Tag and

This strategy often is used to capture intact protein complexes and evaluate protein-protein interactions Search for Epitope Tag Antibodies Find compare and review antibodies to different protein epitope tags Search Genetic fusions with fluorescent proteins enable protein

Protein/Peptide Tags

FLAG epitope tag N-DYKDDDTongWei-C is the first epitope tag designed for fusion proteins and is the only patented tag The multiple polyanionic amino acids in the FLAG tag are less likely to affect the activity of a target protein FLAG-tagged proteins can be recognized or recovered with specific antibodies M1 M3 M5 FLAG has been used in bacteria yeast and mammalian cell systems 3 X FLAG

Epitope Tag Antibodies Reporter Protein Antibodies

Epitope tag antibodies are highly specific monoclonal and polyclonal antibodies for the detection and purification of the tagged proteins Reporter tags are frequently used for detecting protein expression cell tracking and cellular localization experiments

Common Epitope Tags(including sequence) in Protein

This is better known as a reporter gene for expression assays Antibodies allow results to be verified by immunological methods Maltose-Binding Protein (MBP) The size (43 kDa) of this tag contributes to its ability to increase soluble protein production in E coli Along with GST and Thioredoxin it is popular as a 'solubility' tag

LY6E Gene

LY6E (Lymphocyte Antigen 6 Family Member E) is a Protein Coding gene Diseases associated with LY6E include T-Cell Lymphoma Subcutaneous Panniculitis-Like and Acute Promyelocytic Leukemia Among its related pathways are Metabolism of proteins and Ectoderm Differentiation An important paralog of this gene is GPIHBP1

Epitope tagging

The advent of hybridoma technology made monoclonal antibodies available thereby providing a reproducible source of antibody However the first commercially available epitope tags were originally designed for protein purification Examples of these early commercial products include the FLAG 6 His and glutathione-S-transferase (GST) systems The FLAG tagging system in its original version

Anti

The Myc tag (amino acid sequence: EQKLISEEDL) is a popular epitope tag derived from c-myc gene product that can be fused to a protein through recombinant DNA technology The Myc epitope tag contains 10 amino acids and has a molecular weight of 1 2 KDa Anti-Myc Tag antibodies facilitate the detection isolation and purification of Myc-tagged target proteins without a protein-specific

Anti

Anti-NET Antibody solute carrier family 6 member 2 References (1) Recommended Applications Product Description Monoclonal Antibody against Human SLC6A2 Alternative Gene Names SLC6A2 NAT1 NET1 SLC6A5 Open Datasheet Price $445 00 Product Number AMAb91116 Unit Size 100 l Concentration Lot dependent Availability 10 in Stock Ships Ready to Ship Share by E-mail Add to

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Switchable reporter enzymes based on mutually exclusive

18 10 2013Reporter enzymes for two other antibodies (HA-tag and Dengue virus type I) were obtained by simply replacing the epitope sequences This new sensor design represents a modular and generic approach to construct antibody reporter enzymes without the cumbersome optimization required by previous engineering strategies

CRISPR

Western blotting with antibodies to each tag revealed a MYOCD protein product of ≈150 kDa a size considerably larger than that reported in virtually all publications MYOCD protein was most abundant in some adult smooth muscle-containing tissues with surprisingly low-level expression in the heart Both alleles of Myocd are active in aorta because a 2-fold increase in protein was seen in

Tags and Tag antibodies

Antibodies that recognize a tag are commonly called tag antibodies and the tag sequence that serves as an epitope for tag antibodies is called an "epitope tag" Many different types of epitope tags have been developed for different purposes Among them the FLAG (DYKDDDTongWei DDDTongWei etc ) HA His Myc and V5 tags are commonly used because expression cassette vectors for these tags are

Myc Tag Mouse anti

Epitope tags provide a method to localize gene products in a variety of cell types study the topology of proteins and protein complexes identify associated proteins and characterize newly identified low abundance or poorly immunogenic proteins when protein specific antibodies are not available Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag

Epitope Design Peptide Synthesis : ABGENEX :

Epitope Design Peptide Synthesis As an innovative antibody and reagent supplier company ABGENEX provides a full range of custom antibody services for the life science Biomedical industry With over twelve years of antibody experience our scientists have the skills and tools necessary to help you at every step of the process from antigen design to antibody production and validation

AbD Serotec

AbD Serotec is one of the world's leading antibody manufacturers supplying over 15 000 antibodies kits and accessories We pride ourselves on providing great customer support for all the products and services we supply so please contact us with any questions on their use or the results you can expect We can even help source products not currently available or develop them to your

Epitope Tags Reporters

Epitope tags are peptide or protein sequences covalently conjugated usually to the C- or N-terminus of a recombinant protein The addition of epitope tags allows researchers to better identify and purify a target protein within a cell lysate or localize it and assess its expression in intact cells when studying transfectants expressing the fusion protein

Protein tag

T7-tag an epitope tag derived from the T7 major capsid protein of the T7 gene (MASMTGGQQMG) Used in different immunoassays as well as affinity purification Mainly used TC tag a tetracysteine tag that is recognized by FlAsH and ReAsH biarsenical compounds (CCPGCC) Ty tag (EVHTNQDPLD) V5 tag a peptide recognized by an antibody (GKPIPNPLLGLDST)

Tags and Tag Antibodies

However specific antibodies to a target protein are not always available In that case the target protein can be added a marker (tag) by genetic engineering techniques and expressed in the living organism A specific antibody to the tag can distinguish the target protein from other similar proteins

Epitope Tag Reporter Protein Antibodies

Epitope Tag and Reporter Protein Antibodies The use of epitope tags in recombinant DNA techniques allows the detection of proteins where specific antibodies are not available This eliminates the need to generate a new antibody for each protein saving time resources and money Reporter genes (also known as protein expression markers or small reporter proteins) are used in gene expression

Identification of Human Single

Wu et al describe the development of a versatile platform for rapid isolation of fully human single-domain antibodies and apply this methodology to identify SARS-CoV-2-specific antibodies These human single-domain antibodies target diverse epitopes within the SARS-CoV-2 spike protein receptor binding domain (RBD) and may yield potential therapeutic candidates for COVID-19

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